Finnzyme phusion manual

The Phusion™ Site-Directed Mutagenesis Kit contains reagents for a total of 20 mutagenesis reactions (including control reactions), and control plasmid and primers for 10 reactions. Component Amount Storage Phusion™ Hot Start II DNA Polymerase, 2 U/μL 10 µL −20°C 5X Phusion™ HF Buffer mL dNTP Mix 10 mM each 20 µL FastDigest File Size: KB. Phusion DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific. Phusion® is a registered trademark and property of Thermo Fisher Scientific! No equivalent Bio-Rad product; please contact Finnzymes at www.doorway.ru FL DyNAmo Capillary kit, rxns FS Native DyNAzyme DNA polymerase, U FS Phusion high-fidelity DNA polymerase, U iProof high-fidelity DNA polymerase , and FL Phusion high-fidelity DNA polymerase, U.

Specifications. Hot Start. No. Includes. Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide mM MgCl2 in the final 1x concentration. Quantity. U (2U/µL) Concentration. Phusion DNA Polymerase is supplied with standard 5X Phusion HF Buffer, as well as 5X Phusion GC Buffer, which can be used for complex or GC-rich templates. Each of these buffers contains MgCl 2 ( mM at the final [1X] reaction concentration). Reactions can also be optimized using the provided DMSO or MgCl 2 solutions. Finnzymes’ Phusion™ High-Fidelity DNA Polymerase offers extreme performance for all PCR applications. Incorporating an exciting new technology, Phusion DNA Polymerase brings together a novel Pyrococcus-like enzyme with a processivity-enhancing domain. The Phusion DNA Polymerase generates long templates with an accuracy and speed previously.

১৫ নভেম্বর, ২০১৪ Phusion did not amplify a plasmid with complementary primers but used The QuikChangeTM manual recommends continuing with transformation. The polymerases Phusion and Pfu are both fine but the main difference is this: recommended 50°C. For the rest use the parameters of the Phusion manual. ২৮ জানু, ২০২০ WARNING! Read the Safety Data Sheets (SDSs) and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves.